Genome-wide Analysis of Drosophila Circular RNAs Reveals Their Structural and Sequence Properties and Age-Dependent Neural Accumulation

Westholm, Jakub Orzechowski; Miura, Pedro; Olson, Sara; Shenker, Sol; Joseph, Brian; Sanfilippo, Piero; Celniker, S; Graveley, Brenton R.; Lai, Eric C.

doi:10.1016/j.celrep.2014.10.062

Cell Reports

2014

DOI: 10.1016/j.celrep.2014.10.062

|Get access via publisher |Summarize |Cite

|

Sign up to set email alerts

Genome-wide Analysis of Drosophila Circular RNAs Reveals Their Structural and Sequence Properties and Age-Dependent Neural Accumulation

Jakub Orzechowski Westholm

¹

,

Pedro Miura

²

,

Sara Olson

³

et al.

Abstract: Circularization was recently recognized to broadly expand transcriptome complexity. Here, we exploit massive Drosophila total RNA-sequencing data, >5 billion paired-end reads from >100 libraries covering diverse developmental stages, tissues and cultured cells, to rigorously annotate >2500 fruitfly circular RNAs. These mostly derive from back-splicing of protein-coding genes and lack poly(A) tails, and circularization of hundreds of genes is conserved across multiple Drosophila species. We elucidate structural… Show more

Help me understand this report

Cited by 1,107 publications

(1,098 citation statements)

References 37 publications

Supporting

66

Mentioning

1,027

Contrasting

2

Unclassified

2

Order By: Relevance

“…Here, by generating and analyzing deep RNA sequencing data of 164 blood samples collected from the long-lived families, we conducted a genome-wide investigation of circRNA expression patterns in a long-lived Chinese cohort for the first time. Different from the previous observations that circRNAs appear to accumulate with age in the neural tissues of mice [41], Drosophila [42] and Caenorhabditis elegans [43], we found that the overall circRNA expression in the LLIs was significantly lower than that in the younger controls (viz., F1SPs). As circRNAs are expressed in a tissue-specific manner [14] and not easily degraded by nucleases owing to the special loop structure [44], our result indicated that there may be some potential circRNA degradation or generation mechanism in peripheral blood of the longevous individuals.…”

Section: Discussioncontrasting

confidence: 99%

Specific Gain and Loss of Co-Expression Modules in Long-Lived Individuals Indicate a Role of circRNAs in Human Longevity

Ge

¹

,

Jiang

²

,

Yang

³

et al. 2022

Genes

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Here, by generating and analyzing deep RNA sequencing data of 164 blood samples collected from the long-lived families, we conducted a genome-wide investigation of circRNA expression patterns in a long-lived Chinese cohort for the first time. Different from the previous observations that circRNAs appear to accumulate with age in the neural tissues of mice [41], Drosophila [42] and Caenorhabditis elegans [43], we found that the overall circRNA expression in the LLIs was significantly lower than that in the younger controls (viz., F1SPs). As circRNAs are expressed in a tissue-specific manner [14] and not easily degraded by nucleases owing to the special loop structure [44], our result indicated that there may be some potential circRNA degradation or generation mechanism in peripheral blood of the longevous individuals.…”

Section: Discussioncontrasting

confidence: 99%

Specific Gain and Loss of Co-Expression Modules in Long-Lived Individuals Indicate a Role of circRNAs in Human Longevity

Ge

¹

,

Jiang

²

,

Yang

³

et al. 2022

Genes

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…As circRNAs are generally more stable than their linear siblings, the apparent increase in levels of circRNA over age could be due, at least in part, to differences in stability of circular and linear RNAs. These findings are consistent with recent reports showing that select circRNAs are enriched in the nervous system and further regulated by age [ 51 , 54 ].…”

Section: Resultssupporting

confidence: 93%

“…A control cross was set up using a shRNA transgenic line targeting gfp . Consistent with a recent report, we detected higher levels of Edis in flies kept at 29°C than those at 18°C ( S9 Fig ) [ 51 ]. Compared with controls ( tub-Gal80 ts ; da>shGFP ), depletion of Edis in vivo leads to markedly elevated levels of the Dpt transcript under both non-infection and E .…”

Section: Resultssupporting

confidence: 93%

The circular RNA Edis regulates neurodevelopment and innate immunity

Xiong

¹

,

Liang

²

,

Liu

³

et al. 2022

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…1e). In agreement with previous findings46, the splice accepting circularized exon was most commonly exon 2, and we observed a general preference for circRNAs to emanate from exons at the 5′ ends of genes (Fig. 1f).…”

Section: Resultssupporting

confidence: 93%

CircRNA accumulation in the aging mouse brain

Gruner

¹

,

Cortés-López

²

,

Cooper

³

et al. 2016

Sci Rep

Self Cite

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

Scite is an AI-powered platform that helps researchers discover and evaluate scientific literature through Smart Citations, showing whether studies support or contradict a claim. Now part of Research Solutions, Scite has indexed 1.6B+ citations, partners with 30+ publishers, and serves 2M users worldwide.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA