Developmental Cell
 2016
DOI: 10.1016/j.devcel.2016.09.016
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A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization

Fred Etoc
1
,
Jakob J. Metzger
2
,
Albert Ruzo
3
et al.

Abstract: Summary The earliest aspects of human embryogenesis remain mysterious. To model patterning events in the human embryo we used colonies of human embryonic stem cells (hESCs) grown on micropatterned substrate and differentiated with BMP4. These gastruloids recapitulate the embryonic arrangement of the mammalian germ layers and provide an assay to assess the structural and signaling mechanisms patterning the human gastrula. Structurally, high-density hESCs lateralize their TGF-β receptors to their lateral side in… Show more

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Cited by 359 publications

(543 citation statements)
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“…S4C). Furthermore, in accord with previous findings ( 49 ), BMPR1A-Myc or ACVR1B-Myc exhibits a predominantly basolateral localization in cysts expressing DOX-inducible constructs (fig. S4C).…”
Section: Resultssupporting
confidence: 93%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
“…S4C). Furthermore, in accord with previous findings ( 49 ), BMPR1A-Myc or ACVR1B-Myc exhibits a predominantly basolateral localization in cysts expressing DOX-inducible constructs (fig. S4C).…”
Section: Resultssupporting
confidence: 93%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
“…4a ). These results follow previous findings based on micropatterning technology 6 , 7 . The NE differentiation potential of large colonies was confirmed when hESCs were spontaneously differentiated by FGF2 and TGF-β deprivation (Extended Data Fig.…”
Section: Resultssupporting
confidence: 92%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
“…Two-dimensional micropatterned colonies before BMP4 treatment (0 h) expressed POU5F1, SOX2, and NANOG uniformly ( Figures 1 A and 1E). At 12 h of BMP4 treatment, high pSMAD1 staining was restricted to the colony edge ( Figure S1 B), consistent with previous reports ( Etoc et al., 2016 ; Warmflash et al., 2014 ). TE marker GATA3 expression was generally confined to the outer edge, suggesting the initiation of differentiation ( Gunne-Braden et al., 2020 ).…”
Section: Resultssupporting
confidence: 92%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
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