Spatially resolved cell polarity proteomics of a human epiblast model

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Indeed, our data indicate that EFNB2 is not only enriched at the lateral membrane in Caco-2 cell spheroids, but is also essential for organizing proper spheroid morphology. Our observations are also consistent with previous reports of EPHA2 and EPHB4 being expressed at the basolateral membrane in MDCK cells and human pluripotent stem cell cysts, respectively (Harada et al , 2015; Wang et al , 2021). It remains a possibility that EPHRs at the basal domain associate in cis with ephrins, a mode of interaction that was previously detailed in different cellular contexts (Carvalho et al , 2006; Kao & Kania, 2011; Yin et al , 2004; Falivelli et al , 2013).…”

Section: Discussionsupporting

confidence: 93%

EPHA1 and EPHB4 tyrosine kinase receptors regulate epithelial morphogenesis

Lavoie

¹

,

Scribe

²

,

Chartier

³

et al. 2024

Preprint

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…Retraction of ZO-1, observed upon MLCK inhibition, indicated that another factor is responsible for this reversed or basal actomyosin flow. Examining myosin distribution, we noted its presence in both apical and basal regions, consistent with earlier observations in mESCs (Shi et al, 2023) and human embryonic human pluripotent stem cell-derived cysts (Wang et al, 2021). We also observed distinct effects of inhibiting MLCK and ROCK on myosin activity and ZO-1 localization in cells.…”

Section: Discussionsupporting

confidence: 90%

Opposing actomyosin pools generate cortical flows that establish epithelial polarity

Shi

¹

,

Ohler

²

,

Jesus

³

et al. 2024

Preprint

1

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

“…These findings provide key insights into how LE and lysosomal compartments contribute to human epiblast lumen formation and expand the list of machinery required for apicosome formation ( Fig. 8H , (1, 8, 15, 37)).…”

Section: Discussionmentioning

confidence: 65%

“…To do this, we used APEX2-based proximity labeling using doxycycline (DOX)-inducible PODXL constructs C-terminally tagged with APEX2 (PODXL-APEX2), and APEX2 fused to a nuclear export sequence (APEX2-NES) for subsequent background subtraction during ratiometric analysis ( Fig. 2A,B , (15, 24)).…”

Section: Resultsmentioning

confidence: 99%

The endo-lysosomal system drives lumen formation in a human epiblast model

Rengarajan

¹

,

Wang

²

,

Lin

³

et al. 2025

Preprint

Self Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.