Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

Walls, Alexandra C.; Park, Young‐Jun; Tortorici, M. Alejandra; Wall, Abigail; McGuire, Andrew T.; Veesler, David

doi:10.1016/j.cell.2020.02.058

Cell

2020

DOI: 10.1016/j.cell.2020.02.058

|Get access via publisher |Summarize |Cite|

|

Sign up to set email alerts

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

Alexandra C. Walls

¹

,

Young‐Jun Park

²

,

M. Alejandra Tortorici

³

et al.

Abstract: Graphical AbstractHighlights d SARS-CoV-2 uses ACE2 to enter target cells d SARS-CoV-2 and SARS-CoV bind with similar affinities to ACE2 d Structures of SARS-CoV-2 spike glycoprotein in two conformations d SARS-CoV polyclonal antibodies inhibit SARS-CoV-2 spikemediated entry into cellsIn Brief SARS-CoV-2, a newly emerged pathogen spreading worldwide, binds with high affinity to human ACE2 and uses it as an entry receptor to invade target cells.Cryo-EM structures of the SARS-CoV-2 spike glycoprotein in two dist… Show more

Help me understand this report

View preprint versions

Cited by 9,343 publications

(9,693 citation statements)

References 103 publications

Supporting

274

Mentioning

9,239

Contrasting

18

Unclassified

142

Order By: Relevance

“…Extracted particles were subjected to two-dimensional (2D) classification, which resulted in class averages populated with fully closed trimers, wherein all three RBDs are in the ‘down’ position. This finding stands in contrast to previous cryo-EM studies of SARS-CoV-2 spike ectodomains, in which substantial proportions of partially open spikes with at least one RBD in the ‘up’ position were observed [ 57 – 59 ]. A 3.1-Å–resolution 3D reconstruction of the WT SHC014-CoV spike trimer was obtained and found to resemble that of the closed SARS-CoV-2 spike (Figs 4A , S4 and S5 ), which was expected given the ≈80% sequence identity between the proteins.…”

Section: Resultscontrasting

confidence: 99%

Distinct pathways for evolution of enhanced receptor binding and cell entry in SARS-like bat coronaviruses

Tse

¹

,

Acreman

²

,

Ricardo-Lax

³

et al. 2024

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Extracted particles were subjected to two-dimensional (2D) classification, which resulted in class averages populated with fully closed trimers, wherein all three RBDs are in the ‘down’ position. This finding stands in contrast to previous cryo-EM studies of SARS-CoV-2 spike ectodomains, in which substantial proportions of partially open spikes with at least one RBD in the ‘up’ position were observed [ 57 – 59 ]. A 3.1-Å–resolution 3D reconstruction of the WT SHC014-CoV spike trimer was obtained and found to resemble that of the closed SARS-CoV-2 spike (Figs 4A , S4 and S5 ), which was expected given the ≈80% sequence identity between the proteins.…”

Section: Resultscontrasting

confidence: 99%

Distinct pathways for evolution of enhanced receptor binding and cell entry in SARS-like bat coronaviruses

Tse

¹

,

Acreman

²

,

Ricardo-Lax

³

et al. 2024

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…S 6 ). Unlike most structural studies of the apo SARS-CoV-2 S trimer or its complexes where various conformational states corresponding to 0–3 RBDs open were observed, 10 , 12 , 18 , 19 , 26 only one conformational state with all 3 RBDs open was observed in our structure of the S in complex with the cocktail of NAbs (Fig. 5a ).…”

Section: Resultscontrasting

confidence: 97%

Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection

Yao

¹

,

Sun

²

,

Deng

³

et al. 2020

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…The result showed that SARS-CoV-2 and SARS-CoV pseudoviruses entered all three types of target cells with similar efficiency (Fig. 5C), which is consistent with two recent studies (31,34).…”

Section: Resultssupporting

confidence: 92%

Cell entry mechanisms of SARS-CoV-2

Shang

¹

,

Wan

²

,

Luo

³

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

Scite is an AI-powered platform that helps researchers discover and evaluate scientific literature through Smart Citations, showing whether studies support or contradict a claim. Now part of Research Solutions, Scite has indexed 1.6B+ citations, partners with 30+ publishers, and serves 2M users worldwide.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA