Transcriptional landscape of Mycobacterium tuberculosis infection in macrophages

Roy, Sugata; Schmeier, Sebastian; Kaczkowski, Bogumił; Arner, Peter; Alam, Tanvir; Ozturk, Mumin; Tamgue, Ousman; Parihar, Suraj P.; Kawaji, Hideya; Itoh, Masayoshi; Lassmann, Timo; Carninci, Piero; Hayashizaki, Yoshihide; Forrest, Alistair R. R.; Guler, Reto; Bajić, Vladimir B.; Brombacher, Frank; Suzuki, Harukazu

doi:10.1038/s41598-018-24509-6

Sci Rep

2018

DOI: 10.1038/s41598-018-24509-6

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Transcriptional landscape of Mycobacterium tuberculosis infection in macrophages

Sugata Roy¹,

Sebastian Schmeier

²

,

Bogumił Kaczkowski

³

et al.

Abstract: Mycobacterium tuberculosis (Mtb) infection reveals complex and dynamic host-pathogen interactions, leading to host protection or pathogenesis. Using a unique transcriptome technology (CAGE), we investigated the promoter-based transcriptional landscape of IFNγ (M1) or IL-4/IL-13 (M2) stimulated macrophages during Mtb infection in a time-kinetic manner. Mtb infection widely and drastically altered macrophage-specific gene expression, which is far larger than that of M1 or M2 activations. Gene Ontology enrichment… Show more

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Cited by 97 publications

(115 citation statements)

References 43 publications

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“…In contrast, AGK2 treatment significantly affected the gene expression profile induced upon Mtb infection. In total, Mtb infection led to the differential expression of 992 genes which is in agreement with the previous findings ( Mehta et al, 2016 ; Roy et al, 2018 ). Genes that remained unaltered after AGK2 treatment were categorized as SIRT2 independent (414 genes) and the rest as SIRT2 dependent (578 genes) ( Figure 3A ).…”

Section: Resultssupporting

confidence: 92%

Host sirtuin 2 as an immunotherapeutic target against tuberculosis

Bhaskar

¹

,

Kumar

²

,

Khan

³

et al. 2020

Elife

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…In contrast, AGK2 treatment significantly affected the gene expression profile induced upon Mtb infection. In total, Mtb infection led to the differential expression of 992 genes which is in agreement with the previous findings ( Mehta et al, 2016 ; Roy et al, 2018 ). Genes that remained unaltered after AGK2 treatment were categorized as SIRT2 independent (414 genes) and the rest as SIRT2 dependent (578 genes) ( Figure 3A ).…”

Section: Resultssupporting

confidence: 92%

Host sirtuin 2 as an immunotherapeutic target against tuberculosis

Bhaskar

¹

,

Kumar

²

,

Khan

³

et al. 2020

Elife

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…3G ) in infected M1-like macrophages, indicates a high rate of conversion of newly synthesized citrate toward the formation of itaconate in infected macrophages. This result is consistent with high-level induction of Irg1 in infected macrophages ( 31 , 52 ), despite the decrease in overall flux to itaconate from glucose, as discussed above. Taken together, these data demonstrate the rerouting of 13 C glucose carbon flux from the TCA cycle to glycolysis during M1-like polarization.…”

Section: Resultssupporting

confidence: 88%

Glutamine Is Required for M1-like Polarization of Macrophages in Response to Mycobacterium tuberculosis Infection

Jiang

¹

,

Qiu

²

,

Kurland

³

et al. 2022

mBio

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…We determined that there were significantly more genes that were upregulated than downregulated upon Mtb infection in macrophages. This is consistent with previous transcriptome analyses of Mtb infected macrophages (Wu et al 2012;Seshadri et al 2017;Papp et al 2018;Roy et al 2018;Lee et al 2019;Looney et al 2021;Pu et al 2021). We also found that there was almost an equal number of DAR where CA increased or decreased upon Mtb infection in macrophages.…”

Section: Discussionsupporting

confidence: 93%

Transcriptome and chromatin accessibility mapping reveals a type I Interferon response triggered by Mycobacterium tuberculosis infection

Madden

¹

,

Hamra

²

,

Berton

³

et al. 2022

Preprint

0

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

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