2024
DOI: 10.1002/edn3.70023
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The Effect of Spatio‐Temporal Sampling and Biological Replication on the Detection of Kelp Forest Fish Communities Using eDNA Metabarcoding

Abstract: Environmental DNA (eDNA) metabarcoding surveys can support the acquisition of extensive biodiversity data to support ecosystem monitoring and conservation actions. However, the optimization of eDNA metabarcoding project design is essential to capture spatio‐temporal heterogeneity of eDNA signals and maximize diversity detection. In this study, we developed a system‐specific approach to detect fish communities in kelp forests, by analyzing fine‐scale spatio‐temporal patterns in eDNA signals at two sites along t… Show more

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Cited by 9 publications

(17 citation statements)
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“…Previous studies reported the same (Sato et al 2017;Zhang et al 2020;Blabolil et al 2022;Courtaillac et al 2024). Thus, pooled samples generally have lower detection power than individual samples.…”
Section: Sampling Effort For Biodiversity Surveillance In Paddy Fieldssupporting
confidence: 62%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…Previous studies reported the same (Sato et al 2017;Zhang et al 2020;Blabolil et al 2022;Courtaillac et al 2024). Thus, pooled samples generally have lower detection power than individual samples.…”
Section: Sampling Effort For Biodiversity Surveillance In Paddy Fieldssupporting
confidence: 62%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…The intra-site spatiotemporal sampling design of this study was important for accounting for the ecology of eDNA, particularly its transport and persistence (Barnes and Turner 2016), enabling a more comprehensive representation of fish communities at each site. The consistent finding of significant differences in species richness and beta-diversity across intra-site predictors underscores how hierarchical spatiotemporal sampling can effectively detect fine-scale fluctuations in eDNA signals (Courtaillac et al 2024). Further, increasing the number of spatiotemporal sampling points within each site also enhanced observed richness, allowing for the accumulation of larger proportions of expected richness.…”
Section: Building a Fish Biodiversity Baseline For The Gasfmentioning
confidence: 63%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
“…Autumn months (September–November) showed strong similarities, with S. scombrus (Scombridae) and G. flavescens (Gobidae) displaying high read abundance as in (Carvalho et al, 2024), suggesting that these species define late-season assemblages. Following the reanalysis of the first year sampling data, we found no clear dominant species in January 2023, but identified S. scombrus as a dominant species in January 2024 using the MiFish primer set, highlighting how extended sampling can reveal dynamic changes single-year studies might miss (Courtaillac et al, 2024; Zinger et al, 2019).…”
Section: Discussionmentioning
confidence: 93%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.