Single-cell RNA expression profiling of ACE2, the receptor of SARS-CoV-2

Zhao, Yu; Zhao, Zixian; Wang, Yujia; Zhou, Yueqing; Ma, Yu; Zuo, Wei

doi:10.1101/2020.01.26.919985

2020

DOI: 10.1101/2020.01.26.919985

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Single-cell RNA expression profiling of ACE2, the receptor of SARS-CoV-2

Yu Zhao,

Zixian Zhao,

Yujia Wang

et al.

Abstract: A novel coronavirus (2019-nCov) was identified in Wuhan, Hubei Province, China in December of 2019. This new coronavirus has resulted in thousands of cases of lethal disease in China, with additional patients being identified in a rapidly growing number internationally. 2019-nCov was reported to share the same receptor, Angiotensin-converting enzyme 2 (ACE2), with SARS-Cov.Here based on the public database and the state-of-the-art single-cell RNA-

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Cited by 1,036 publications

(1,018 citation statements)

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“…Alveolar epithelial type 2 (AT2), ciliated and club cells emerged as the cells with the highest expression of both receptors (Fig 1A; Fig S1A). These observations are consistent with published studies demonstrating that ACE2 is indeed expressed highest in AT2 and ciliated cells (Jia et al, 2005;Mulay et al, 2020;Zhao et al, 2020). In a cohort of deceased COVID-19 patients, we observed by H&E ( Fig S1B) that gas-exchanging flattened AT1 pneumocytes are virtually replaced by cuboidal cells that were subsequently confirmed to be AT2-like cells via immunofluorescent staining with the AT2-specific marker, surfactant protein-C (SFTPC; Fig 1B upper panel; Fig S1C; top).…”

Section: Resultssupporting

confidence: 93%

Adult Stem Cell-derived Complete Lung Organoid Models Emulate Lung Disease in COVID-19

Tindle

¹

,

Fuller

²

,

Fonseca

³

et al. 2020

Preprint

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…Alveolar epithelial type 2 (AT2), ciliated and club cells emerged as the cells with the highest expression of both receptors (Fig 1A; Fig S1A). These observations are consistent with published studies demonstrating that ACE2 is indeed expressed highest in AT2 and ciliated cells (Jia et al, 2005;Mulay et al, 2020;Zhao et al, 2020). In a cohort of deceased COVID-19 patients, we observed by H&E ( Fig S1B) that gas-exchanging flattened AT1 pneumocytes are virtually replaced by cuboidal cells that were subsequently confirmed to be AT2-like cells via immunofluorescent staining with the AT2-specific marker, surfactant protein-C (SFTPC; Fig 1B upper panel; Fig S1C; top).…”

Section: Resultssupporting

confidence: 93%

Adult Stem Cell-derived Complete Lung Organoid Models Emulate Lung Disease in COVID-19

Tindle

¹

,

Fuller

²

,

Fonseca

³

et al. 2020

Preprint

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…The ACE2 expressed cells were significantly enriched in CRC samples compared with CRA and NC samples (5.15% vs 2.94% and 1.75%, p<0.05) (Fig.2 B, C). In consistent with previous studies( 9, 10 ), we also observed that ACE2 cell were mainly expressed in epithelial cells which highly express EPCAM(Fig.2D).…”

Section: Resultssupporting

confidence: 93%

Profiling ACE2 expression in colon tissue of healthy adults and colorectal cancer patients by single-cell transcriptome analysis

Chen

¹

,

Xuan

²

,

Yan

³

et al. 2020

Preprint

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…We found that the majority of ACE2-expressing cells in normal human lung tissues were AT2 cells ( SFTPC -positive) (Fig. 1A and 1C), which is consistent with a previous study 7 . Unexpectedly, ACE2 was mainly expressed in arterial vascular cells, but rarely in AT2 in fibrotic lung tissues (Fig.…”

Section: Resultssupporting

confidence: 92%

Single-cell RNA Analysis on ACE2 Expression Provides Insight into SARS-CoV-2 Blood Entry and Heart Injury

Guo

¹

,

Wei

²

,

Li

³

et al. 2020

Preprint

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

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