Journal of General Virology
 2021
DOI: 10.1099/jgv.0.001506
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Micro-fusion inhibition tests: quantifying antibody neutralization of virus-mediated cell–cell fusion

Nazia Thakur
1
,
Carina Conceicao
2
,
Ariel Isaacs
3
et al.

Abstract: Although enveloped viruses canonically mediate particle entry through virus–cell fusion, certain viruses can spread by cell–cell fusion, brought about by receptor engagement and triggering of membrane-bound, viral-encoded fusion proteins on the surface of cells. The formation of pathogenic syncytia or multinucleated cells is seen in vivo, but their contribution to viral pathogenesis is poorly understood. For the negative-strand paramyxoviruses respiratory syncytial virus (RSV) and Nipah virus (NiV), cell–cell … Show more

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Cited by 32 publications

(35 citation statements)
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“…To elucidate the potential mechanism of action of the PHIs on the RSV life cycle, we examined RSV-F driven cell-cell fusion using a split GFP-luciferase reporter cell-cell fusion system, which uses a doxycylcine inducible expression of RSV-F in an effector cell line, combined with a target cell line stably expressing the corresponding half of the reporter ( Fig.3a ) 43 . Treatment with Daprodustat and Molidustat resulted in a dose-dependent inhibition of RSV-F cell-cell fusion with estimated IC 50 values of 11 and 42 µM, respectively ( Fig.3b ).…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
“…To elucidate the potential mechanism of action of the PHIs on the RSV life cycle, we examined RSV-F driven cell-cell fusion using a split GFP-luciferase reporter cell-cell fusion system, which uses a doxycylcine inducible expression of RSV-F in an effector cell line, combined with a target cell line stably expressing the corresponding half of the reporter ( Fig.3a ) 43 . Treatment with Daprodustat and Molidustat resulted in a dose-dependent inhibition of RSV-F cell-cell fusion with estimated IC 50 values of 11 and 42 µM, respectively ( Fig.3b ).…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
show abstract
“…Fusion assays are based on direct morphological criteria (Braga et al, 2021) or on reporter gene (luciferase, green fluorescent protein (GFP), β-galactosidase) complementation, either by using split genes (Buchrieser et al, 2021;Kandeel et al, 2021;Plaper et al, 2021;Rajah et al, 2021;Thakur et al, 2021;Zhang et al, 2021) or by segregation of transactivator and promoter (Zhao et al, 2021a;Hörnich et al, 2021). In this study, we opted for the phage T7 promoter-luciferase reporter gene complementation system because luciferase assay is rapid, objective, quantitative, and very sensitive with a wide linear range of at least 7 orders of magnitude (Supplementary Figure S1).…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
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“…We furthermore explored the modulation of spike-mediated membrane fusion as a potential neutralization mechanism, using established cell-cell fusion assays 36 . W25 and its derivatives (Fc-fusion and monomeric Fc-fusion [FcM]) caused extensive cell-cell fusion for SARS-CoV2 D614G-, Beta- and Omicron-infected cells, demonstrated by luciferase activity (Fig.…”
Section: Resultsmentioning
confidence: 99%
Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.
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