Extreme heterogeneity of influenza virus infection in single cells

Russell, Alistair B.; Trapnell, Cole; Bloom, Jesse D.

doi:10.7554/elife.32303

Elife

2018

DOI: 10.7554/elife.32303

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Extreme heterogeneity of influenza virus infection in single cells

Alistair B. Russell

¹

,

Cole Trapnell

²

,

Jesse D. Bloom

³

Abstract: Viral infection can dramatically alter a cell’s transcriptome. However, these changes have mostly been studied by bulk measurements on many cells. Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection. We find extremely wide cell-to-cell variation in the productivity of viral transcription – viral transcripts comprise less than a percent of total mRNA in many infected cells, but a few cells derive over half their mRNA from virus. Some infected cells fa… Show more

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Cited by 323 publications

(368 citation statements)

References 109 publications

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306

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“…By contrast, influenza infection on its own resulted in up-regulation of a small set of 7 genes (>2-fold, Fig 6F) including only a few interferon-stimulated genes. This observation is consistent with recent work showing that activation of immune pathways by influenza virus is rare at the single cell level during infections with viruses that have relatively few defective particles [63].…”

Section: Resultssupporting

confidence: 93%

Comprehensive profiling of translation initiation in influenza virus infected cells

Machkovech

¹

,

Bloom

²

,

Subramaniam

³

2019

Self Cite

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…By contrast, influenza infection on its own resulted in up-regulation of a small set of 7 genes (>2-fold, Fig 6F) including only a few interferon-stimulated genes. This observation is consistent with recent work showing that activation of immune pathways by influenza virus is rare at the single cell level during infections with viruses that have relatively few defective particles [63].…”

Section: Resultssupporting

confidence: 93%

Comprehensive profiling of translation initiation in influenza virus infected cells

Machkovech

¹

,

Bloom

²

,

Subramaniam

³

2019

Self Cite

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…The normalized and log 10 transformed counts of viral transcripts per cell were then compared across conditions. We found that the amount of detected GFHK99 viral mRNA varies widely between individual DF-1 cells, which is consistent with previous observations 38–40 . In contrast, GFHK99 viral mRNA levels are uniformly low in MDCK cells, especially at the lower MOIs tested ( Figure 6A , left facet).…”

Section: Resultssupporting

confidence: 93%

Collective interactions augment influenza A virus replication in a host-dependent manner

Phipps

¹

,

Ganti

²

,

Jacobs

³

et al. 2019

Preprint

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…4B, cells infected with a high MOI contained more viral UMIs/cell that those infected with a low MOI, supporting the hypothesis that multiple infections per cell result in increased level of viral proteins. Interestingly, and in accordance with previous reports (43)(44)(45), we found great heterogeneity in the numbers of viral transcripts across the cells in infected cultures for both MOIs.…”

Section: Resultssupporting

confidence: 93%

Innate Immune Response to Influenza Virus at Single-Cell Resolution in Human Epithelial Cells Revealed Paracrine Induction of Interferon Lambda 1

Ramos

¹

,

Smith

²

,

Ruf-Zamojski

³

et al. 2019

J Virol

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

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