Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules

Markmiller, Sebastian; Soltanieh, Sahar; Server, Kari L.; Mak, Raymond; Jin, Wenhao; Fang, Mark Y.; Luo, En‐Ching; Krach, Florian; Yang, Dejun; Sen, Anindya; Wozniak, Jacob M.; Gonzalez, David J.; Kankel, Mark W.; Gao, Fen‐Biao; Bennett, Eric J.; Lécuyer, Éric; Yeo, G

doi:10.1016/j.cell.2017.12.032

Cell

2018

DOI: 10.1016/j.cell.2017.12.032

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Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules

Sebastian Markmiller

¹

,

Sahar Soltanieh

²

,

Kari L. Server

³

et al.

Abstract: Stress granules (SGs) are transient ribonucleoprotein (RNP) aggregates that form during cellular stress and are increasingly implicated in human neurodegeneration. To study the proteome and compositional diversity of SGs in different cell types and in the context of neurodegeneration-linked mutations, we used ascorbate peroxidase (APEX) proximity labeling, mass spectrometry, and immunofluorescence to identify ∼150 previously unknown human SG components. A highly integrated, pre-existing SG protein interaction … Show more

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Cited by 917 publications

(1,092 citation statements)

References 55 publications

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“…5). These results are consistent with previous studies on the influence of R-DPRs on stress granule assembly [24][25][26] and the presence of UPF1 in stress granules 29 , raising the possibility that NMD inhibition may be due to the sequestration of UPF1 and other NMD factors in stress granules.…”

Section: Resultssupporting

confidence: 92%

C9orf72 arginine-rich dipeptide repeats inhibit UPF1-mediated RNA decay via translational repression

Sun

¹

,

Eshov

²

,

Zhou

³

et al. 2020

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…5). These results are consistent with previous studies on the influence of R-DPRs on stress granule assembly [24][25][26] and the presence of UPF1 in stress granules 29 , raising the possibility that NMD inhibition may be due to the sequestration of UPF1 and other NMD factors in stress granules.…”

Section: Resultssupporting

confidence: 92%

C9orf72 arginine-rich dipeptide repeats inhibit UPF1-mediated RNA decay via translational repression

Sun

¹

,

Eshov

²

,

Zhou

³

et al. 2020

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Consistent with previous results, DHX36 is mostly cytoplasmic, and upon arsenite stress, DHX36 is enriched in stress granules as indicated by colocalization with PABPC1, and therefore could affect RNA structures within stress granules (Supplementary Fig. 3C) (22, 29, 30).…”

Section: Resultssupporting

confidence: 91%

Impact of G-tract RNAs and the DHX36 helicase on stress granule composition and formation

Cheng

¹

,

Ripin

²

,

Cech

³

et al. 2025

Preprint

0

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…It demonstrates a conspicuous increasing trend in all seven community importance metrics, ranging from low-probability to high-probability ( i.e., prediction probability from 0.5-1.0) RNA granule proteins. The observation aligns with previous experimentally identified RNA granule cores in a dense PPI network 7 , identified in specific experimental environments 7 . Notably, the high-confidence already known RNA granule proteins candidates comprise up to only 33% of proteins from prediction probability from 0.5-0.6 to 0.8-0.9, as listed in Table S2 .…”

Section: Resultssupporting

confidence: 91%

Unravelling biomolecular and community grammars of RNA granules via machine learning

Ban

¹

,

Yan

²

,

Dawson

³

2024

Preprint

0

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

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