Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity

Silva, Thushan I. de; Gould, Victoria; Mohammed, Nuredin; Cope, Alethea; Meijer, Adam; Zutt, Ilse; Reimerink, Johan; Kampmann, Beate; Höschler, Katja; Zambon, Maria; Tregoning, John S.

doi:10.1016/j.jim.2017.06.008

Journal of Immunological Methods

2017

DOI: 10.1016/j.jim.2017.06.008

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Comparison of mucosal lining fluid sampling methods and influenza-specific IgA detection assays for use in human studies of influenza immunity

Thushan I. de Silva

¹

,

Victoria Gould

²

,

Nuredin Mohammed

³

et al.

Abstract: We need greater understanding of the mechanisms underlying protection against influenza virus to develop more effective vaccines. To do this, we need better, more reproducible methods of sampling the nasal mucosa. The aim of the current study was to compare levels of influenza virus A subtype-specific IgA collected using three different methods of nasal sampling. Samples were collected from healthy adult volunteers before and after LAIV immunization by nasal wash, flocked swabs and Synthetic Absorptive Matrix … Show more

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Cited by 34 publications

(25 citation statements)

References 24 publications

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“…Normalization of SARS-CoV-2 WT-RBD IgA using total IgA further reduced the variability in serially collected samples, which is consistent with the study of de Silva et al. ( 26 ).…”

Section: Discussionsupporting

confidence: 89%

Exploring the standardized detection and sampling methods of human nasal SARS-CoV-2 RBD IgA

Zhang

¹

,

Jia

²

,

Hu

³

et al. 2025

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Normalization of SARS-CoV-2 WT-RBD IgA using total IgA further reduced the variability in serially collected samples, which is consistent with the study of de Silva et al. ( 26 ).…”

Section: Discussionsupporting

confidence: 89%

Exploring the standardized detection and sampling methods of human nasal SARS-CoV-2 RBD IgA

Zhang

¹

,

Jia

²

,

Hu

³

et al. 2025

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…This was the first study to use SAM strips to collect samples for metabolomic analysis. The high yield of metabolites recovered by SAM strips reflects our recent experience with detecting virus-specific antibody where the SAM strips have significantly more concentrated sample recovery (de Silva et al, 2017). Interestingly in another study viral loads in nasosorption were several fold lower than in nasopharyngeal aspirate (NPA), another dilutional technique with variable yield (Thwaites et al, 2017).…”

Section: Discussionmentioning

confidence: 87%

Comparative Metabolomic Sampling of Upper and Lower Airways by Four Different Methods to Identify Biochemicals That May Support Bacterial Growth

Farne

¹

,

Groves

²

,

Gill

³

et al. 2018

Front. Cell. Infect. Microbiol.

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Nasosorption is noninvasive and does not require rotation within the nostril, providing individuals with a more comfortable experience . Moreover, nasosorption using SAM strips enable the collection of more concentrated samples for analysis . The most critical step for NLF measurement is the elution of NLF from the SAM.…”

Section: Resultsmentioning

confidence: 99%

“…Several bioanalytical considerations were factored into the development of a PK assay for NLF. Collection of NLF has traditionally been performed using nasal washes or nasal swabs, which made it difficult to obtain suitable recovery of analytes, particularly where concentrations of target analytes are very low . Nasal swabs also require rotation within the nostril, which can be uncomfortable .…”

Section: Resultsmentioning

confidence: 99%

“…Collection of NLF has traditionally been performed using nasal washes or nasal swabs, which made it difficult to obtain suitable recovery of analytes, particularly where concentrations of target analytes are very low. 10 Nasal swabs also require rotation within the nostril, which can be uncomfortable. 16 Nasosorption is noninvasive and does not require rotation within the nostril, providing individuals with a more comfortable experience.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Multiplex Hybrid Antigen-Capture LC-MRM Quantification in Sera and Nasal Lining Fluid of AZD7442, a SARS-CoV-2-Targeting Antibody Combination

Mu

¹

,

Huang

²

,

Bouquet

³

et al. 2022

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

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