CDC20B is required for deuterosome-mediated centriole production in multiciliated cells

Revinski, Diego R.; Zaragosi, Laure‐Emmanuelle; Boutin, Camille; Ruiz-Garcia, Sandra; Deprez, Marie; Thomé, Virginie; Rosnet, Olivier; Gay, Anne-Sophie; Mercey, Olivier; Paquet, Agnès; Pons, Nicolas; Ponzio, Gilles; Marcet, Brice; Kodjabachian, Laurent; Barbry, Pascal

doi:10.1038/s41467-018-06768-z

Nat Commun

2018

DOI: 10.1038/s41467-018-06768-z

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CDC20B is required for deuterosome-mediated centriole production in multiciliated cells

Diego R. Revinski

¹

,

Laure‐Emmanuelle Zaragosi

²

,

Camille Boutin

³

et al.

Abstract: Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, … Show more

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Cited by 79 publications

(71 citation statements)

References 57 publications

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“…Control morphant MCCs displayed >90% of centriole disengagement ( Fig. 5, B and C ), which is in line with a previous report ( Revinski et al, 2018 ). However, >75% of CEP97 morphant MCCs and >85% of Dyrk1a morphant MCCs failed to complete centriole disengagement ( Fig.…”

Section: Resultssupporting

confidence: 93%

CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis

Lee

¹

,

Nagashima

²

,

Yoon

³

et al. 2021

Journal of Cell Biology

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…Control morphant MCCs displayed >90% of centriole disengagement ( Fig. 5, B and C ), which is in line with a previous report ( Revinski et al, 2018 ). However, >75% of CEP97 morphant MCCs and >85% of Dyrk1a morphant MCCs failed to complete centriole disengagement ( Fig.…”

Section: Resultssupporting

confidence: 93%

CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis

Lee

¹

,

Nagashima

²

,

Yoon

³

et al. 2021

Journal of Cell Biology

Get access via publisher Add to dashboard Cite

Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…We first analyzed with confocal microscopy the respective distribution of Deup1 and Centrin signals in deuterosomal cells. Consistent with our previously published observations (Revinski et al, 2018), the deuterosomes of both A6-MCI MCCs and Xenopu s epidermal MCCs showed irregular shapes and sizes (Fig.4A, B). We then analyzed the distribution of γ-Tubulin and Pericentrin, two proteins that we had previously identified as being associated with the deuterosome of Xenopus epidermal and mouse ependymal MCCs (Revinski et al, 2018).…”

Section: Resultssupporting

confidence: 92%

“…Consistent with our previously published observations (Revinski et al, 2018), the deuterosomes of both A6-MCI MCCs and Xenopu s epidermal MCCs showed irregular shapes and sizes (Fig.4A, B). We then analyzed the distribution of γ-Tubulin and Pericentrin, two proteins that we had previously identified as being associated with the deuterosome of Xenopus epidermal and mouse ependymal MCCs (Revinski et al, 2018). Confocal immunofluorescence confirmed that those proteins localized to A6-MCI deuterosomes associated to Centrin-positive centrioles (Fig.4C, D).…”

Section: Resultssupporting

confidence: 92%

Deciphering the dynamic proteome of multiciliated cells

Boutin

¹

,

Rosnet

²

,

Touret

³

et al. 2025

Preprint

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

“…9 ) in which emi2 is required during MCC differentiation to inhibit APC/C cdh1 , leading to the phosphorylation and activation of plk1, which, in turn, acts in a graded manner to promote multiple steps in centriole amplification and basal body formation. This model is consistent with previous studies implicating plk1 in MCC differentiation ( 38 ) and with the role of plk1 in multiple steps of the centrosome cycle in dividing cells ( 29 – 31 , 39 ).…”

Section: Discussionsupporting

confidence: 93%

Emi2 enables centriole amplification during multiciliated cell differentiation

Kim

¹

,

Chien

²

,

Ryan

³

et al. 2022

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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